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[2004] found that a combination of TGF- 1, LIF, FGF-2, and bronectin in a synthetic serum replacement induced pluripotent expansion of preexisting hES cell lines Similarly, Xu et al [2005] found that a combination of the bone morphogenetic protein (BMP) antagonist Noggin and FGF-2 in serum replacement and on Matrigel was similarly potent in maintaining preexisting hES cell lines in a pluripotent state, whereas other groups have found that FGF-2 synergizes with activin or Nodal in serum-free chemically de ned medium to retain pluripotency [Vallier et al, 2005] Pebay et al [2005] discovered that a combination of sphingosine-1-phosphate with platelet-derived growth factor (PDGF) could promote hES cell expansion in completely de ned medium, but this still required propagation of cells on Matrigel Ludwig et al [2006] recently reported the derivation of two new hES cell lines under feeder-free conditions with a fully disclosed chemically de ned medium.

Figure 8-18: Declaring a stroke through the New Style dialog box stores the stroke definition for easy access from all Fireworks documents.

However, one of the cell lines had an abnormal chromosomal complement (XXY) when analyzed after 4 months in culture, and the other cell line become trisomic for chromosome 12 after 7 months, suggesting that this medium formulation may not be completely competent to support long-term normal hES cell proliferation Whether any or all of these formulations will permit derivation of new cell lines that remain karyotypically normal still remains to be determined..

FIGURE 28.10 The Trap Presets panel (left) and the New Trap Preset dialog box (right)

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Being able to move through a document easily is a key skill in Illustrator. Rarely can you fit an Dragging: Press the mouse button and keep it pressed while you move the mouse. You entire illustration in the document window at a sufficient magnification to see much of the image s drag the cursor to choose in, zooming out, or moving off to the side, above, text, move detail. Usually, you re zoomingitems from menus, select contiguous characters of or below to focus objects, and create marquees on certain areas of the document. (dotted rectangles used for zooming the view).

The Erase Mode option both controls and limits what and how the Eraser Tool erases. As shown in Figure 5-31, the Erase Mode pop-up reveals five options: Erase Normal, Erase Fills, Erase Lines, Erase Selected Lines, and Erase Inside. These function in a similar manner to the Brush Mode options: Erase Normal: With this, the Eraser Tool functions like a normal eraser. It erases all lines and fills that it passes over, as long as they are on the active layer. Erase Fills: In Erase Fills Mode, the Eraser Tool becomes a specialty eraser, erasing only fills and leaving lines unaffected. Erase Lines: When in Erase Lines Mode, the Eraser Tool works by erasing lines only and leaving fills unaffected. Erase Selected Fills: In Erase Selected Fills Mode, the Eraser Tool becomes even more specialized. In this mode, it only erases fills that are currently selected, leaving unselected fills and all lines unaffected. Erase Inside: With Erase Inside Mode, the Eraser Tool only erases the area of fill on which you initiate erasing. This is much like the Erase Selected Fills Mode, except that the selection is accomplished with the initial erasure. In this mode, the eraser leaves all other fills and all lines unaffected.

When you need to saturate colors selectively, it s not always necessary to make selections or to create masks and then spend time refining them. You can do so by taking the following steps: 1. Create a Hue/Saturation Adjustment layer above your image layer. 2. Next, select the color that you want to saturate from the Edit pop-up menu in the Hue/Saturation dialog box. Many users miss the controls available via the Hue/Saturation command, possibly because when they enter the dialog box, the eyedroppers and the color range slider controls are grayed out. However, as soon as you select a color from the Edit pop-up menu, a default color range is defined for you and the eyedroppers and the color range sliders spring to life (Figure 12-11).

Figure 10-16: It s easy to group items into new fields and customize the name of the items in the group.

atalyst projects are imported into Flash Builder 4, where you or a Flex developer add the necessary coding to create connections to back-end services, add additional ActionScript, and otherwise finalize the project. The finalized project is compiled into an SWF for viewing in Flash Player. You may already be familiar with SWF files, as it is the same file type that results from publishing a movie in Flash Professional. A Catalyst or Flex project is really nothing more than a Flash movie, and just like Flash movies created in Flash Professional, these projects can contain multimedia assets.

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