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An ef cient BCCH deployment is critical in the narrowband scenarios. Owing to the nature of the GSM BCCH channel, GSM operators have traditionally reserved 15 frequencies (3 MHz) or more for the BCCH TRX deployment. This means that in the case of 3.6-MHz deployment (18 frequencies), only three frequencies would remain for additional transceivers. Not only does this limit the number of additional TRXs deployed but also seriously impacts the performance of the hopping layer since the frequency diversity gains would be substantially limited. One option to enhance hopping layer performance is to tighten the BCCH reuse to release more frequency channels to become available for the hopping layer. However, the performance of BCCH layer may get degraded and since the traf c served by the BCCH TRX is likely to be a relatively high percentage of the total amount of traf c carried by the cell, this may have a signi cant impact on the overall performance. Therefore, for narrowband scenarios, in order to obtain the maximum spectral ef ciency with the spectrum allocated, both BCCH and hopping layer performance have to be very carefully optimised. In order to benchmark the performance of the network, the overall bad quality samples (BQS) need to be considered. If a certain outage T of BQS or unsatis ed users is allowed in a network, in order to see the maximum capacity achievable in the network, the effect of BCCH performance can be expressed as follows: BQSBCCH x + BQShopping (1 x) = T where BQSBCCH is the outage of BQS for BCCH TRXs BQShopping is the outage of BQS for hopping TRXs

Click the New Graphic Style button to make your selected object s attributes into a new style in the Graphic Styles panel. You can also create a new style by choosing New Graphic Style from the popup menu in the Graphic Styles panel. To create a new style, follow these steps:

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Finally, an alternative to the use of animal serum for hES propagation is the use of various synthetic serum replacements, for example, Invitrogen s Knock-Out Serum Replacer Many groups have shown that hES lines can be established and propagated in this medium [see, eg, Lysdahl et al, 2006; Simon et al, 2005; Inzunza et al, 2005; Wang et al, 2005], but the exact formulation of this and related reagents is generally unobtainable, they are very expensive, many still contain animal products, and either mouse or human feeders or feeder-conditioned media are still required to maintain pluripotency However, the optimal conditions for generating and expanding hES cells to the numbers required for research and therapeutic applications will require these cells to be grown with relatively simple culture conditions, in the absence of feeders, serum additives, and extracellular matrix substrates.

FIGURE 2-4: You ll need to compensate for Netscape 4 s inability to properly apply inheritance rules.

Unlike hES, a number of mouse ES cell lines can be grown on gelatin-coated plates without feeder layers, as long as suf cient concentrations of leukemia inhibitory factor (LIF) are provided Human ES cells do not proliferate in the presence of LIF alone, and so identi cation of the mitogens and growth factors secreted by the broblast and other feeder cells that maintain hES pluripotency will be required However, despite a number of biochemical and molecular approaches to identify these factors [eg, Lim and Bodnar, 2002], to date the identity of the pluripotency growth factor(s) remains elusive, although Pyle et al [2006] have shown that MEFs secrete neurotropins that seem to support pluripotent expansion of hES cells, even in the absence of MEFs Nevertheless, some progress in identifying chemically de ned media that support the propagation of hES cell has been made.

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An extracellular matrix (ECM) such as Matrigel [Xu et al, 2001] or laminin and conditioned medium from MEFs or human broblasts can be used to propagate hES cells under feeder-free culture conditions This reduces carryover of feeder cells for protocols that may require the cells to be feeder-free, such as for karyotyping or differentiation studies Yao et al [2006] showed that hES cell lines previously derived on MEFs could subsequently be propagated feeder-free on Matrigel in de ned medium containing N2 and B27 neuronal stem cell supplements and 20 ng/mL FGF-2 The limitation of this approach is that the B27 supplement is of unknown composition and Matrigel is derived from a mouse tumor cell line, so this still represents a xenogeneic system Amit et al.

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